题目：Structural Studies indicate Possible Different Interactions between KCNE1 and KCNE 2 in Their Association and Modulation of KCNQ1 Channel

主讲人：田长麟 中国科学技术大学教授

邀请人：丁久平 教授

时间：2011年4月6日  星期三  上午10:00-11:00

地点：8797威尼斯老品牌2楼会议室

摘要：Members of KCNE family are membrane proteins with single transmembrane helix. They are all found to associate and modulate alpha-subunit of different potassium channels. KCNQ1 is a voltage gated delayed outward rectified potassium channel and have very diverse but essential functions in brain and cardiovascular system. KCNE1 and KCNE2 are known to associate with KCNQ1 but have different modulation influences. Three dimensional structure of KCNE1 and KCNE2 have been determined in the presence of detergent micelles, using solution NMR. Some local differences have been observed through examing structure of KCNE1 and KCNE2. Furthermore, computation and physiological studies indicate that KCNE1 and KCNE2 have different dynamic regulation on KCNQ1 potassium conductance.

主讲人简介：

田长麟 教授，博士生导师。2003年5月在美国佛罗里达州立大学获得生物物理博士学位。2003年到2006年在美国范德比尔特大学结构生物学中心作博士后研究。

 主要研究兴趣：

主要研究方向为应用多种方法和技术手段研究与疾病相关的膜蛋白（主要是受体和离子通道蛋白），结核杆菌外膜蛋白的结构和动力学，主要的研究手段有液体核磁共 振、电子顺磁共振、X-射线晶体学和X射线小角散射等技术。同时结合其他化学生物学、生物物理学方法如非天然氨基酸、全自动膜片钳以及受体药理学等开展膜 蛋白的结构和功能研究。目前已经在Science, JACS, Biochemistry，FASEB Journal等国际一流杂志上发表了独立研究和合作研究的相关工作。

  主要代表论文:   

1)       Van Horn WD, Kim HJ, Ellis CD, Hadziselimovic A, Sulistijo ES, Karra MD, Tian C, S?nnichsen FD, Sanders CR Solution Nuclear Magnetic Resonance Structure of Membrane Integral Diacylglycerol Kinase Science 2009 324(5935): 1726-1729

2)       Kang C*, Tian C*,Sonnichsen FD, Smith JA, Meiler J, George AL Jr, Vanoye CG, Kim HJ, Sanders CR. Structure of KCNE1 and Implications for How It Modulates the KCNQ1 Potassium Channel. Biochemistry. 2008 Aug 5;47(31):7999-8006（* 同等贡献作者）

3)       Tian, C., Vanoye, C.G., Kang, C., Welch, R.C., Kim, H.J., George, A.L., Sanders, C.R. Preparation, Functional Characterization, and NMR Studies of Human KCNE1, a Voltage-Gated Potassium Channel Accessory Subunit Associated With Deafness and Long QTSyndrome Biochemistry 2007 July; 46:11459-11472

4)       Tian C, Breyer RM, Kim HJ, Karra MD, Friedman DB, Karpay A, Sanders CR., Solution NMR spectroscopy of the human vasopressin v2 receptor, a G protein-coupled receptor J Am Chem Soc. 2005 Jun.127(22):8010-80101

5)       Tian, C., Karra M.D., Ellis C.D., Jacob, J., Sonnichsen, F.D., Sanders, C.R., Membrane protein sample preparation for TROSY NMR screening. Methods in Enzymology 2005 394:321-34

6)       Tian, C., Gao, F., Pinto, L.H., Lamb, R.A., Cross, T.A., Initial structural characterization of the M2 amphipathic helices in lipid bilayers. Protein Sci. 2003 Nov;12(11):2597-605. 

7)   Tian, C., Tobler, K., Lamb, R.A., Pinto, L.H., Cross, T.A., Expression and initial structural insights from solid state NMR of the M2 proton channel from influenza A virus. Biochemistry 2002 Sep 17;41(37):11294-11300