题目： "Regulations of Synaptic Vesicle Priming and Fusion by Munc13-1 and Synaptotagmin-1" 

主讲人：美国德州大学西南医学中心  马聪博士

报告时间：2011年4月11日（周一） 上午9:30

报告地点：生命学院二楼会议室

主讲人简介：

EDUCATION

Huazhong University of Science & Technology

Institute of Biophysics, Chinese Academy of Sciences Sept 2004 to Aug 2007

Ph.D in Biophysics, Lab of Dr. Tao Xu, Beijing, China

Huazhong University of Science & Technology Sept 2000 to Jun 2003

Masters of Environmental Biology, Lab of Dr. Kaihong Zhao, Wuhan, China

South-Central University for Nationalities Sept 1996 to Jun 2000

Bachelor of Science in Biochemistry Wuhan, China

EXPERIENCE

Postdoctoral Research, Lab of Dr. Jose Rizo-Rey, Sept 2007 to Present

UT Southwestern Medical Center, Dallas, TX

 Expressed, purified, and studied membrane proteins and protein complexes involved in chemical synaptic

transmission using biochemically and biophysics.

 Studied the 150kDa Snare complex/Munc18-1/Munc13-1 complex interaction by multidimensional NMR.

 Used fluorescence anisotropy and FRET to probe protein-protein, protein-membrane interactions.

 Studied calcium-induced structure conformation changes in PICK1 structure CD spectroscopy.

 Developed a Ca2+ and Synaptotagmin-1 regulated membrane fusion assay.

 Established a time based FRET assay both in solution and on membranes to monitor SNARE complex

formation by different regulators.

 Solved the structure of the Munc13-1 MUN C-terminal domain.

 Expressed and purified Munc13-1 in bacuvirus expression system.

Ph.D Research, lab of Dr. Tao Xu, Sept 2004 to Aug 2007

Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

 Separated, purified and identified GLUT4 (Glucose Transporter 4) using tissue homogenization,

differential centrifugation/density gradient centrifugation, ion-exchange purification and Western blot.

 Expressed, purified and crystallized the critical domains of Munc13-1.

 Expressed, purified and crystallized Exocyst complex component Sec10.

 Expressed, purified and crystallized GPCR protein.

Visiting Scholar, Lab of Dr. Hugo Scheer, Sept 2003 to Feb 2004

University of Munich & Max Planck Institute of Biochemistry, Munich, Germany

 Crystallized PecE/PecF complex.

 Studied the PecE/PecF catalyzed addition/isomerization of PCB-PVB to PecA.

SKIL LS

 NMR, X-ray crystallography.

 PCR, site-specific mutagenesis, tissue extraction, western blot, pull-down, co-immunoprecipitation.

 Membrane protein expression and purification; in vitro membrane protein reconstitution.

 Lipid mixing and liposome-protein co-flotation assays.

 Nanodisc-reconstituted assembly of membrane protein complexes.

 Protein labeling, fluorescence quenching and anisotropy, FRET, CD and Micro fluidic assays.

 E.coli, Sf9 cell and HEK293 expression.

PUBL ICATIONS

Munc13 mediates the transition from the closed Syntaxin/Munc18 complex to the SNARE complex.

Ma C*, Li W*, XU Y, Rizo J. Nat Struct Mol Biol. (accepted) * Co-first author

Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.

Citri A*, Bhattacharyya S*, Ma C, Morishita W, Rizo J and Malenka R C. J Neurosci. 2010 Dec 8;30(49):

16437-52 * Co-first author

Remote homology between Munc13 MUN domain and vesicle tethering complexes.

Pei J, Ma C, Rizo J, Grishin NV. J Mol Biol. 2009 Aug 21;391(3):509-17.

The Janus-faced Nature of the C(2)B Domain is Fundamental for Synaptotagmin-1 Function.

Xue M*, Ma C*, Craig TK, Rosenmund C, Rizo J. Nat Struct Mol Biol. 2008 Nov;15(11):1160-8. * Co-first

author

An efficient co-expression and purification system for the complex of Stx4 and C-terminal domain of Synip.

Tian W, Ma C, Liu Y, Xu T. Biochem Biophys Res Commun. 2008 Jul 4;371(3):366-70.

Expression, purification and characterization of critical domains of Munc13-1.

Ma C, Hou H, Tian W, Xu T. Acta Biochim Biophys Sin. 2007 Aug; 39(8):617-23.