学术报告：Translation control exerted by the cold-shock protein YB-1: molecular and cellular approaches
报告人：David Pastré
时间：2016.07.08上午9:00-11:00
地点：东十一楼221
摘要：Translation is tightly regulated in mammalian cells for keeping adequate protein levels. This function is notably accomplished by mRNA-binding proteins recognizing a specific set of mRNAs to repress or facilitate their translation. Here I will consider YB-1, a mRNA-binding protein involved in tumorogenesis. Our results indicate that YB-1 binds to mRNA cooperatively which leads to a preferential multimerization on specific mRNAs. Based on this model and cellular data, we also found that YB-1 can play an important role in the translational response to stress and notably in stress granule dynamics. Finally I will introduce a novel technology developed in my laboratory to detect and quantify direct or indirect protein interactions by fluorescence microscopy in living mammalian cells using microtubules as platforms. I will show how we probed the cooperative binding of YB-1 to mRNA in the cytoplasm.

个人简介：
After studying physics and optics at the University of Montpellier, David Pastré developed at the time of his thesis (1996-1999) a set up to collect cathodoluminescence in near field. During a postdoctoral fellowship at the University of Virginia (2000-2001), he designed a method to observe living mammalian cells at high-resolution with a scanning ion conductance microscope. As a teacher-researcher at the University of Evry, he deciphered the mechanisms leading to DNA absorption on mica and studied the formation of DNA and RNA/protein complexes on mica by atomic force microscopy.
David Pastré is now at the head of the SABNP laboratory (INSERM unit U1204) and professor at the University of Evry. He is currently investigating, at the cellular and molecular levels, the dynamics and structure of RNA/protein complexes involved in the control of protein synthesis and the mechanisms which regulate microtubule dynamics. He also continues to develop novel methods to explore cellular and molecular processes.
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